The ability to measure 7,000 proteins at once starts with exquisite specificity
The SomaScan [Assay] also has a two-step approach to specificity that is sometimes forgotten by non-experts…between these two steps, the assay turns out to be quite highly specific.”
By using uniquely modified DNA with hydrophobic functional groups, our SOMAmer® reagents achieve a greater degree of shape matching, enabling discernment between nearly identical proteins.
SOMAmer reagents are selected for slow off-rates when correctly bound to their intended target to ensure that correct proteins remain preferentially bound during rigorous washing.
Polyanionic competitors are used to universally prevent rebinding of transient, non-specific interactions. There is no such universal competitor for antibodies.
X-ray crystal structure of a SOMAmer reagent bound to PDGF-BB.
Modifications to the bases are shown in purple, and the DNA backbone and unmodified bases are in teal.
Typical dissociation for a SOMAmer reagent
This graph shows the typical dissociation for a SOMAmer reagent over time, in this case targeting C3.