Technical and Support Documentation

In-depth technical information about SOMAmer reagents, SOMAscan, sample handling, and nonhuman samples.

White Papers

SOMAscan Proteomic Assay Technical White Paper
While traditional proteomics tools have limitations in biomarker discovery, the SOMAscan assay is an aptamer-based binding assay that complements your current methods.

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Technical White Paper SOMAmer Reagent Specificity
SOMAmer reagents bind to their target proteins with affinities comparable to and often exceeding those of antibodies. In addition, SOMAmer reagents also engage their targets in a highly specific manner. This white paper summarizes some of the existing evidence that, collectively, provides rationale for the exquisite specificity of SOMAmer reagents.

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Technical Notes

Data Standardization Short Note
Standardization is an important tool for removing systematic biases from raw assay data. This document summarizes four techniques used to standardize SOMAscan assay results.

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SOMAmer Technical Note Rev3 (2015)
The interrogation of proteomes in a highly multiplexed and efficient manner remains a coveted and challenging goal in biology and medicine. We introduce an entirely new way to bind proteins, the SOMAmer® reagent, as an evolutionary leap forward in protein recognition. These high-affinity protein-binding reagents are very specific and allow for extremely high multiplexing of protein measurements in a high-throughput and reproducible manner with very small sample volume requirements. SOMAmer reagents form the backbone of our SOMAscan assay platform and represent a powerful new method for biomarker discovery.

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Nonhuman Samples in the SOMAscan Assay, Technical Note (2015)
Many SOMAmer reagents generated to pure human proteins cross-react with non-human orthologs and therefore can be used in SOMAscan to identify deferential expression of these analytes in non-human samples. We have evaluated the signaling of the SOMAscan assay by interrogating mouse plasma, rat plasma and cerebrospinal fluid (CSF), and cat and dog serum and plasma by the methods described in this technical note.

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Verification and Validation of SOMAscan Assay 1.3k
The SOMAscan Assay is optimized for protein biomarker discovery. Both the individual SOMAmer reagents included in the assay and the assay itself are validated for the relative quantification of human protein levels in endogenous matrix. This technical notes describes the verification and validation of the SOMAscan Assay 1.3k.

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Application Notes

Histochemical Staining of Tissues with SOMAmer Reagents
This Application Note discusses the suitability of SOMAmer reagents in histochemical staining of tissues.

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SOMAmer Antagonists of Protein Activity
This Application Note discusses the suitability of SOMAmer reagents in inhibiting the activities of protein targets.

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Polyanionic Competition to Reduce Non-Specific Binding of SOMAmer Reagents
This application note illustrates the use of a SomaLogic® Polyanionic Competitor (P/N 910-00001) as a universal SOMAmer reagent competitor.

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SOMAmer-Antibody Sandwich ELISA Assay
This application note illustrates the protocols used of SOMAmer reagents as capture and detection reagents. Either method can be done on plates or on beads.

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Performing Protein Affinity Purification with SOMAmer Reagents
This application note provides an example of a repeated purification of a target using a SOMAmer reagent column.

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Protein Capture for Mass Spectrometry and SDS PAGE
This application note discusses using SOMAmer reagents in mass spectrometry and SDS Page. For a SOMAmer reagent capture assay, SOMAmer reagents are pre-immobilized on streptavidin agarose beads and incubated with purified protein and/or matrix.

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SOMAmer Reagents in Flow Cytometry Assays
This application note discusses using SOMAmer reagents in flow cytometry.

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Labeling Cell Surface Receptors for Analysis by Flow Cytometry Using SOMAmer Reagents

This application note describes the use of SOMAmer reagents for measuring, by flow cytometry, the cell surface expression levels of three receptors implicated in cancer biology: Epidermal growth factor receptor (EGFR or ERBB1), Receptor tyrosine kinase erbB2 (ERBB2), and Programmed cell death 1 ligand 1 (PD-L1 or B7-H1).

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Protein Detection by Fluorescent Cell Microscopy using SOMAmer Reagents

This application note describes the use of SOMAmer reagents for observing, by fluorescent cell microscopy, the cell surface expression of two receptor tyrosine kinases implicated in cancer biology: Epidermal growth factor receptor (EGFR or ERBB1) and Receptor tyrosine kinase erbB2 (ERBB2).

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Other Documentation

Recommended Sample Handling and Processing (2016)
Recommended procedures for the collection and preparation of samples for assay on SOMAscan at SomaLogic.

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